---
name: majiayu000-opentrons-protocol-agent
description: "Generates executable Python protocols for Opentrons OT-2 and Flex robots from natural language descriptions."
license: "MIT"
metadata:
  decimalai-skill-id: "bfda7bbb-1a1c-4bc1-93c7-c95edd1f2d50"
  decimalai-version-id: "0269806c-be18-405e-bed4-792668641149"
  decimalai-version: "1"
  decimalai-body-sha256: "15b3a981aae14d55995a3b6baa8b3df64e3f6b9ed47740a9f95c2fe3cf89b3b3"
  decimalai-name: "majiayu000/opentrons-protocol-agent"
  decimalai-format: "1"
  decimalai-invocation: "model"
  decimalai-category: "science"
  decimalai-source: "https://app.decimal.ai/s/majiayu000-opentrons-protocol-agent@1/SKILL.md"
---
# Opentrons Protocol Agent

The **Opentrons Protocol Agent** bridges the gap between experimental design and physical execution by translating instructions into Opentrons Python API scripts.

## When to Use This Skill

*   When you need to automate a liquid handling task (PCR prep, ELISA, serial dilution).
*   When you have a text description of a lab protocol and need code for the robot.
*   To validate if a requested protocol is feasible with available labware.

## Core Capabilities

1.  **Protocol Generation**: Creates valid `.py` files for OT-2/Flex.
2.  **Labware Validation**: Checks compatibility of plates and tipracks.
3.  **Volume Calculation**: Automates liquid transfer math (e.g., dilution factors).

## Workflow

1.  **Describe**: Provide the experiment details (source, destination, volumes).
2.  **Generate**: The agent uses the `opentrons_protocol_template.py` or internal logic to draft the script.
3.  **Review**: The user (or a simulator agent) reviews the code for safety.

## Example Usage

**User**: "Create a protocol for 1:2 serial dilution in a 96-well plate."

**Agent Action**:
(Uses an LLM to fill in the template or generate code)
```bash
# Agent writes the file to disk
python3 -m opentrons.simulate generated_protocol.py
```