{"slug":"mims-harvard-tooluniverse-molecular-cloning","source_name":"mims-harvard/tooluniverse-molecular-cloning","name":"Mims Harvard/Tooluniverse Molecular Cloning","description":"Molecular cloning assembly design — Gibson Assembly (overlap design for seamless multi-fragment joining) and Golden Gate Assembly (Type IIS / BsaI / BbsI design with unique 4-bp fusion overhangs). Use when you need to plan how to join DNA fragments into a construct, design assembly overlaps/overhangs, or decide between cloning methods. Covers the domestication (internal-site removal), overhang-uniqueness, and overlap-Tm rules. For PCR primers to generate the fragments, see tooluniverse-primer-de","version":1,"lift":{"pass_rate_delta_pts":27.27,"pass_rate_pct":95.5,"total_cases":22,"passed_cases":21,"tokens_delta_pct":18.1,"turns_delta_pct":0,"verdict":"mixed","benchmark_model":"gemini-3.6-flash","grading_method":"judged","completed_at":"2026-08-06T16:00:47.281588+00:00"},"skill_score":0.9545,"benchmark_models":[{"model":"gemini-3.6-flash","headline":true,"delta_pts":27.27,"with_pass_pct":95.5,"without_pass_pct":68.2,"tokens_delta_pct":18.1,"turns_delta_pct":0,"total_cases":22,"cases_aggregated":21,"verdict":"mixed","never_hurt":true,"completed_at":"2026-08-06T16:00:47.281588+00:00","run_id":"4ae55888-98ea-4c7c-b9fc-adaa35e1de44","version_number":1,"is_latest_version":true,"gate":null}],"trust":{"skill_safety":"passed","safety_status":"clean","intent_verdict":"safe","content_status":"clean","indexable":true},"license":"Apache-2.0","install_count":0,"manifest_hash":"7c130bc14f2baac6087e49719ac43cd6f9bf2e2ea90e361a704a06663ee20a7f","raw_url":"https://app.decimal.ai/s/mims-harvard-tooluniverse-molecular-cloning/SKILL.md","scorecard_url":"https://app.decimal.ai/skills/mims-harvard-tooluniverse-molecular-cloning"}