{"slug":"mims-harvard-tooluniverse-primer-design","source_name":"mims-harvard/tooluniverse-primer-design","name":"Mims Harvard/Tooluniverse Primer Design","description":"PCR / qPCR primer and oligo design — design forward/reverse primers for a target region (SantaLucia nearest-neighbor thermodynamics), compute melting temperature (Tm) and annealing temperature (Ta), check GC content, and screen an oligo for hairpins and primer-dimers. Use when you need primers for a sequence, want to QC an existing primer pair, or need the Tm of an oligo. Covers the primer-design rules (Tm matching, GC clamp, 3'-end, length) and the tools' constraint quirks.","version":1,"lift":{"pass_rate_delta_pts":4.55,"pass_rate_pct":63.6,"total_cases":22,"passed_cases":14,"tokens_delta_pct":63.5,"turns_delta_pct":0,"verdict":"mixed","benchmark_model":"gemini-3.6-flash","grading_method":"judged","completed_at":"2026-08-06T16:13:45.394439+00:00"},"skill_score":0.6364,"benchmark_models":[{"model":"gemini-3.6-flash","headline":true,"delta_pts":4.55,"with_pass_pct":63.6,"without_pass_pct":59.1,"tokens_delta_pct":63.5,"turns_delta_pct":0,"total_cases":22,"cases_aggregated":18,"verdict":"mixed","never_hurt":false,"completed_at":"2026-08-06T16:13:45.394439+00:00","run_id":"952483ae-00b9-445b-9892-fdbbddd0eaee","version_number":1,"is_latest_version":true,"gate":null}],"trust":{"skill_safety":"passed","safety_status":"clean","intent_verdict":"safe","content_status":"clean","indexable":true},"license":"Apache-2.0","install_count":0,"manifest_hash":"254d0bae1900f486c32cc250a68ac2116002290833a784cb259198fc5bfb9a75","raw_url":"https://app.decimal.ai/s/mims-harvard-tooluniverse-primer-design/SKILL.md","scorecard_url":"https://app.decimal.ai/skills/mims-harvard-tooluniverse-primer-design"}