{"slug":"tooluniverse-molecular-cloning","source_name":"tooluniverse-molecular-cloning","name":"Tooluniverse Molecular Cloning","description":"Molecular cloning assembly design — Gibson Assembly (overlap design for seamless multi-fragment joining) and Golden Gate Assembly (Type IIS / BsaI / BbsI design with unique 4-bp fusion overhangs). Use when you need to plan how to join DNA fragments into a construct, design assembly overlaps/overhangs, or decide between cloning methods. Covers the domestication (internal-site removal), overhang-uniqueness, and overlap-Tm rules. For PCR primers to generate the fragments, see tooluniverse-primer-de","version":3,"lift":{"pass_rate_delta_pts":13.04,"pass_rate_pct":73.9,"total_cases":23,"passed_cases":17,"tokens_delta_pct":71.5,"turns_delta_pct":0,"verdict":"mixed","benchmark_model":"gemini-3.6-flash","grading_method":"judged","completed_at":"2026-08-09T16:00:14.232776+00:00"},"skill_score":0.7391,"benchmark_models":[{"model":"gemini-3.6-flash","headline":true,"delta_pts":13.04,"with_pass_pct":73.9,"without_pass_pct":60.9,"tokens_delta_pct":71.5,"turns_delta_pct":0,"total_cases":23,"cases_aggregated":23,"verdict":"mixed","never_hurt":false,"completed_at":"2026-08-09T16:00:14.232776+00:00","run_id":"c8086474-19bd-48cd-8331-fd42533b80d0","version_number":2,"is_latest_version":false,"gate":null}],"trust":{"skill_safety":"passed","safety_status":"clean","intent_verdict":"safe","content_status":"clean","indexable":true},"license":"Apache-2.0","install_count":0,"manifest_hash":"71eace321a98369223200d544b5a792d639591ef9d593bfdf86b6a54b4dab533","raw_url":"https://app.decimal.ai/s/tooluniverse-molecular-cloning/SKILL.md","scorecard_url":"https://app.decimal.ai/skills/tooluniverse-molecular-cloning"}