{"slug":"tooluniverse-primer-design","source_name":"tooluniverse-primer-design","name":"Tooluniverse Primer Design","description":"PCR / qPCR primer and oligo design — design forward/reverse primers for a target region (SantaLucia nearest-neighbor thermodynamics), compute melting temperature (Tm) and annealing temperature (Ta), check GC content, and screen an oligo for hairpins and primer-dimers. Use when you need primers for a sequence, want to QC an existing primer pair, or need the Tm of an oligo. Covers the primer-design rules (Tm matching, GC clamp, 3'-end, length) and the tools' constraint quirks.","version":1,"lift":{"pass_rate_delta_pts":36.36,"pass_rate_pct":40.9,"total_cases":22,"passed_cases":9,"tokens_delta_pct":53.3,"turns_delta_pct":0,"verdict":"mixed","benchmark_model":"gemini-3.6-flash","grading_method":"judged","completed_at":"2026-07-27T22:21:48.285655+00:00"},"skill_score":0.4091,"benchmark_models":[{"model":"gemini-3.6-flash","headline":true,"delta_pts":36.36,"with_pass_pct":40.9,"without_pass_pct":4.5,"tokens_delta_pct":53.3,"turns_delta_pct":0,"total_cases":22,"cases_aggregated":18,"verdict":"mixed","never_hurt":false,"completed_at":"2026-07-27T22:21:48.285655+00:00","run_id":"0dbc25bf-b1a6-492d-bd96-0128abb08dce","version_number":1,"is_latest_version":true,"gate":null}],"trust":{"skill_safety":"passed","safety_status":"clean","intent_verdict":null,"content_status":"clean","indexable":true},"license":"Apache License 2.0","install_count":0,"manifest_hash":"254d0bae1900f486c32cc250a68ac2116002290833a784cb259198fc5bfb9a75","raw_url":"https://app.decimal.ai/s/tooluniverse-primer-design/SKILL.md","scorecard_url":"https://app.decimal.ai/skills/tooluniverse-primer-design"}