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Spatial Deconvolution

Estimate cell type composition in spatial spots using scRNA-seq references.

Required Imports

python
import scanpy as sc
import anndata as ad
import numpy as np
import pandas as pd
import matplotlib.pyplot as plt

Overview

Deconvolution estimates cell type proportions in each spatial spot using a reference single-cell dataset. Essential for Visium data where spots contain multiple cells.

Using cell2location

python
import cell2location
from cell2location.utils.filtering import filter_genes
from cell2location.models import RegressionModel

# Load reference scRNA-seq
adata_ref = sc.read_h5ad('reference_scrna.h5ad')
adata_ref.obs['cell_type'] = adata_ref.obs['cell_type'].astype('category')

# Load spatial data
adata_vis = sc.read_h5ad('spatial_data.h5ad')

# Find shared genes
intersect = np.intersect1d(adata_vis.var_names, adata_ref.var_names)
adata_ref = adata_ref[:, intersect].copy()
adata_vis = adata_vis[:, intersect].copy()

Train Reference Signature Model

python
# Select genes for deconvolution
selected = filter_genes(adata_ref, cell_count_cutoff=5, cell_percentage_cutoff2=0.03,
                        nonz_mean_cutoff=1.12)
adata_ref = adata_ref[:, selected].copy()

# Prepare reference
cell2location.models.RegressionModel.setup_anndata(
    adata_ref,
    labels_key='cell_type',
)

# Train reference model
mod = RegressionModel(adata_ref)
mod.train(max_epochs=250, use_gpu=True)

# Export reference signatures
adata_ref = mod.export_posterior(adata_ref, sample_kwargs={'num_samples': 1000})
ref_sig = adata_ref.varm['means_per_cluster_mu_fg']

Run Spatial Deconvolution

python
# Ensure spatial data has same genes
adata_vis = adata_vis[:, adata_ref.var_names].copy()

# Setup spatial data
cell2location.models.Cell2location.setup_anndata(adata_vis)

# Train deconvolution model
mod_spatial = cell2location.models.Cell2location(
    adata_vis,
    cell_state_df=ref_sig,
    N_cells_per_location=10,  # Expected cells per spot
    detection_alpha=20,
)
mod_spatial.train(max_epochs=30000, use_gpu=True)

# Export results
adata_vis = mod_spatial.export_posterior(adata_vis, sample_kwargs={'num_samples': 1000})

Access Deconvolution Results

python
# Cell type abundances stored in obsm
abundances = adata_vis.obsm['q05_cell_abundance_w_sf']
print(f'Cell types: {abundances.shape[1]}')

# Convert to proportions
proportions = abundances / abundances.sum(axis=1, keepdims=True)
adata_vis.obsm['cell_type_proportions'] = proportions

# Add dominant cell type
cell_types = adata_ref.obs['cell_type'].cat.categories
adata_vis.obs['dominant_cell_type'] = cell_types[proportions.argmax(axis=1)]

Using Tangram (Alternative)

python
import tangram as tg

# Load data
adata_sc = sc.read_h5ad('reference_scrna.h5ad')
adata_sp = sc.read_h5ad('spatial_data.h5ad')

# Preprocess
sc.pp.normalize_total(adata_sc)
sc.pp.log1p(adata_sc)

# Find marker genes
sc.tl.rank_genes_groups(adata_sc, groupby='cell_type', method='wilcoxon')
markers = sc.get.rank_genes_groups_df(adata_sc, group=None)
markers = markers[markers['pvals_adj'] < 0.01].groupby('group').head(100)
marker_genes = markers['names'].unique().tolist()

# Prepare for Tangram
tg.pp_adatas(adata_sc, adata_sp, genes=marker_genes)

# Map single cells to spatial locations
ad_map = tg.map_cells_to_space(
    adata_sc,
    adata_sp,
    mode='clusters',
    cluster_label='cell_type',
    device='cuda:0',
)

# Get cell type proportions
tg.project_cell_annotations(ad_map, adata_sp, annotation='cell_type')
# Results in adata_sp.obsm['tangram_ct_pred']

Using RCTD (via R)

python
# RCTD runs in R; use rpy2 for integration
import rpy2.robjects as ro
from rpy2.robjects import pandas2ri
pandas2ri.activate()

# Save data for R
adata_vis.write_h5ad('spatial_for_rctd.h5ad')
adata_ref.write_h5ad('reference_for_rctd.h5ad')

# R code for RCTD
r_code = '''
library(spacexr)
library(Seurat)

# Load data (convert from h5ad first)
# ... R-specific loading code ...

# Create RCTD object
rctd <- create.RCTD(puck, reference, max_cores=4)
rctd <- run_RCTD(rctd, doublet_mode='full')

# Get results
results <- rctd@results
weights <- normalize_weights(results$weights)
'''

Visualize Cell Type Proportions

python
# Plot cell type abundances spatially
cell_types_to_plot = ['T_cell', 'Macrophage', 'Epithelial', 'Fibroblast']

fig, axes = plt.subplots(2, 2, figsize=(12, 12))
for ax, ct in zip(axes.flatten(), cell_types_to_plot):
    ct_idx = list(adata_ref.obs['cell_type'].cat.categories).index(ct)
    adata_vis.obs[f'{ct}_proportion'] = proportions[:, ct_idx]
    sc.pl.spatial(adata_vis, color=f'{ct}_proportion', ax=ax, show=False,
                  title=ct, cmap='Reds', vmin=0, vmax=1)
plt.tight_layout()
plt.savefig('cell_type_proportions.png', dpi=150)

Pie Chart Per Spot (Advanced)

python
from matplotlib.patches import Wedge

def plot_pie_spatial(adata, proportions, cell_types, spot_size=0.5):
    fig, ax = plt.subplots(figsize=(12, 12))
    colors = plt.cm.tab20(np.linspace(0, 1, len(cell_types)))

    coords = adata.obsm['spatial']
    for i in range(adata.n_obs):
        x, y = coords[i]
        props = proportions[i]
        start_angle = 0
        for j, prop in enumerate(props):
            if prop > 0.01:  # Skip tiny proportions
                wedge = Wedge((x, y), spot_size * 50, start_angle,
                             start_angle + prop * 360, color=colors[j])
                ax.add_patch(wedge)
                start_angle += prop * 360

    ax.set_xlim(coords[:, 0].min() - 100, coords[:, 0].max() + 100)
    ax.set_ylim(coords[:, 1].min() - 100, coords[:, 1].max() + 100)
    ax.set_aspect('equal')
    ax.invert_yaxis()

    # Legend
    handles = [plt.Rectangle((0, 0), 1, 1, color=colors[i]) for i in range(len(cell_types))]
    ax.legend(handles, cell_types, loc='upper right')
    plt.savefig('pie_chart_spatial.png', dpi=150)

Evaluate Deconvolution Quality

python
# Check correlation between expected and observed cell counts
# (if you have known cell type markers)

marker_genes = {
    'T_cell': ['CD3D', 'CD3E', 'CD4', 'CD8A'],
    'Macrophage': ['CD68', 'CD14', 'CSF1R'],
    'Epithelial': ['EPCAM', 'KRT8', 'KRT18'],
}

for ct, markers in marker_genes.items():
    available_markers = [m for m in markers if m in adata_vis.var_names]
    if available_markers:
        marker_expr = adata_vis[:, available_markers].X.mean(axis=1)
        ct_idx = list(cell_types).index(ct)
        ct_prop = proportions[:, ct_idx]
        corr = np.corrcoef(marker_expr.flatten(), ct_prop)[0, 1]
        print(f'{ct}: marker-proportion correlation = {corr:.3f}')

Compare Deconvolution Methods

python
# Store results from different methods
adata_vis.obsm['cell2location'] = cell2location_proportions
adata_vis.obsm['tangram'] = tangram_proportions

# Correlation between methods
for ct_idx, ct in enumerate(cell_types):
    c2l = adata_vis.obsm['cell2location'][:, ct_idx]
    tg = adata_vis.obsm['tangram'][:, ct_idx]
    corr = np.corrcoef(c2l, tg)[0, 1]
    print(f'{ct}: cell2location vs tangram = {corr:.3f}')

Export Results

python
# Save proportions as CSV
prop_df = pd.DataFrame(
    proportions,
    index=adata_vis.obs_names,
    columns=cell_types
)
prop_df.to_csv('cell_type_proportions.csv')

# Save annotated AnnData
adata_vis.write_h5ad('spatial_deconvolved.h5ad')

Related Skills

• spatial-data-io - Load spatial data
• single-cell/data-io - Load scRNA-seq reference
• spatial-visualization - Visualize deconvolution results
• single-cell/markers-annotation - Annotate reference cell types

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